All Stories

  1. Affinity isolation and biochemical characterization of N-degron ligands using the N-recognin, ClpS

    Article • January 2023, Elsevier

    Dr. David A Dougan

  2. Novel modification by L/F-tRNA-protein transferase (LFTR) generates a Leu/N-degron ligand in Escherichia coli

    Article • July 2021, Cold Spring Harbor Laboratory Press

    Dr. David A Dougan

  3. ClpXP-mediated Degradation of the TAC Antitoxin is Neutralized by the SecB-like Chaperone in Mycobacterium tuberculosis

    Article • Journal of Molecular Biology, March 2021, Elsevier

    Dr. David A Dougan

  4. Polymerase delta-interacting protein 38 (PDIP38) modulates the stability and activity of the mitochondrial AAA+ protease CLPXP

    Article • Communications Biology, November 2020, Springer Science + Business Media

    Dr. David A Dougan

  5. Exploring a potential Achilles heel of Mycobacterium tuberculosis : defining the ClpC1 interactome

    Article • FEBS Journal, June 2020, Wiley

    Dr. David A Dougan

  6. PDIP38 is a novel adaptor-like modulator of the mitochondrial AAA+ protease CLPXP

    Article • May 2020, Cold Spring Harbor Laboratory Press

    Dr. David A Dougan

  7. Insight into the RssB-Mediated Recognition and Delivery of σs to the AAA+ Protease, ClpXP

    Article • Biomolecules, April 2020, MDPI AG

    Dr. David A Dougan

  8. Molecular and structural insights into an asymmetric proteolytic complex (ClpP1P2) from Mycobacterium smegmatis

    Article • Scientific Reports, December 2019, Springer Science + Business Media

    Dr. David A Dougan

  9. The Direct Molecular Target for Imipridone ONC201 Is Finally Established

    Article • Cancer Cell, May 2019, Elsevier

    Dr. David A Dougan

  10. N‐degron specificity of chloroplast ClpS1 in plants

    Article • FEBS Letters, April 2019, Wiley

    Dr. David A Dougan

  11. Perrault syndrome type 3 caused by diverse molecular defects in CLPP

    Article • Scientific Reports, August 2018, Springer Science + Business Media

    Dr. David A Dougan

  12. The structural biology of mitochondrial respiratory complex assembly

    Article • Acta Crystallographica Section A Foundations and Advances, August 2018, International Union of Crystallography

    Dr. David A Dougan

  13. Dysregulating ClpP: From Antibiotics to Anticancer?

    Article • Cell Chemical Biology, August 2018, Elsevier

    Dr. David A Dougan

  14. Understanding the Pro/N-end rule pathway

    Article • Nature Methods, April 2018, Springer Science + Business Media

    Dr. David A Dougan

  15. Crystal structure of bacterial succinate:quinone oxidoreductase flavoprotein SdhA in complex with its assembly factor SdhE

    Article • Proceedings of the National Academy of Sciences, March 2018, Proceedings of the National Academy of Sciences

    Dr. David A Dougan

  16. Pupylation of PafA or Pup inhibits components of the Pup-Proteasome System

    Article • FEBS Letters, December 2017, Wiley

    Dr. David A Dougan

  17. AAA+ Machines of Protein Destruction in Mycobacteria

    Article • Frontiers in Molecular Biosciences, July 2017, Frontiers

    Dr. David A Dougan

  18. Pro(moting) the Turnover of Gluconeogenic Enzymes by a New Branch of the N-end Rule Pathway

    Article • Trends in Biochemical Sciences, April 2017, Elsevier

    Dr. David A Dougan

  19. The N-end rule adaptor protein ClpS from Plasmodium falciparum exhibits broad substrate specificity

    Article • FEBS Letters, September 2016, Wiley

    Dr. David A Dougan

  20. LON is the master protease that protects against protein aggregation in human mitochondria through direct degradation of misfolded proteins

    Article • Scientific Reports, December 2015, Nature

    Dr. David A Dougan

  21. Anti-adaptors use distinct modes of binding to inhibit the RssB-dependent turnover of RpoS (σS) by ClpXP

    Article • Frontiers in Molecular Biosciences, April 2015, Frontiers

    Dr. David A Dougan

  22. Mitochondrial matrix proteostasis is linked to hereditary paraganglioma: LON-mediated turnover of the human flavinylation factor SDH5 is regulated by its interaction with SDHA

    Article • The FASEB Journal, January 2014, Federation of American Societies For Experimental Biology (FASEB)

    Dr. David A Dougan

  23. Control of Protein Function through Regulated Protein Degradation: Biotechnological and Biomedical Applications

    Article • Journal of Molecular Microbiology and Biotechnology, January 2013, Karger Publishers

    Dr. David A Dougan

  24. Machines of Destruction – AAA+ Proteases and the Adaptors That Control Them

    Article • January 2013, Springer Science + Business Media

    Dr. David A Dougan

  25. Proteolytic Regulation of Stress Response Pathways in Escherichia coli

    Article • January 2013, Springer Science + Business Media

    Dr. David A Dougan

  26. Regulated Proteolysis in Microorganisms

    Article • January 2013, Springer Science + Business Media

    Dr. David A Dougan

  27. Substrate recognition and processing by a Walker B mutant of the human mitochondrial AAA+ protein CLPX

    Article • Journal of Structural Biology, August 2012, Elsevier

    Dr. David A Dougan

  28. The N-end rule pathway: From recognition by N-recognins, to destruction by AAA+proteases

    Article • Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, January 2012, Elsevier

    Dr. David A Dougan

  29. Unfolded protein responses in bacteria and mitochondria: A central role for the ClpXP machine

    Article • IUBMB Life, October 2011, Wiley

    Dr. David A Dougan

  30. Chemical Activators of ClpP: Turning Jekyll into Hyde

    Article • Chemistry & Biology, September 2011, Elsevier

    Dr. David A Dougan

  31. The bacterial N-end rule pathway: expect the unexpected

    Article • Molecular Microbiology, March 2010, Wiley

    Dr. David A Dougan

  32. Diverse functions of mitochondrial AAA+ proteins: protein activation, disaggregation, and degradationThis paper is one of a selection of papers published in this special issue entitled 8th International Conference on AAA Proteins and has undergone the ...

    Article • Biochemistry and Cell Biology, February 2010, Canadian Science Publishing

    Dr. David A Dougan

  33. Adapting the machine: adaptor proteins for Hsp100/Clp and AAA+ proteases

    Article • Nature Reviews Microbiology, August 2009, Nature

    Dr. David A Dougan

  34. Modification of PATase by L/F-transferase generates a ClpS-dependent N-end rule substrate in Escherichia coli

    Article • The EMBO Journal, May 2009, Wiley

    Dr. David A Dougan

  35. Structural basis of N-end rule substrate recognition in Escherichia coli by the ClpAP adaptor protein ClpS

    Article • EMBO Reports, May 2009, Wiley

    Dr. David A Dougan

  36. Structural basis of N‐end rule substrate recognition in Escherichia coli by the ClpAP adaptor protein ClpS

    Article • EMBO Reports, April 2009, EMBO

    Dr. David A Dougan

  37. Conserved residues in the N‐domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding

    Article • FEBS Journal, February 2008, Wiley

    Professor Bernd G Bukau, Dr. David A Dougan

  38. The tyrosine kinase McsB is a regulated adaptor protein for ClpCP

    Article • The EMBO Journal, March 2007, Wiley

    Dr. David A Dougan

  39. Adaptor protein controlled oligomerization activates the AAA+ protein ClpC

    Article • The EMBO Journal, March 2006, Springer Science + Business Media

    Professor Bernd G Bukau, Dr. David A Dougan

  40. ClpS is an essential component of the N-end rule pathway in Escherichia coli

    Article • February 2006

    Professor Bernd G Bukau, Dr. David A Dougan

  41. Thermotolerance Requires Refolding of Aggregated Proteins by Substrate Translocation through the Central Pore of ClpB

    Article • Cell, November 2004, Elsevier

    Professor Bernd G Bukau, Dr. David A Dougan

  42. Broad yet high substrate specificity: the challenge of AAA+ proteins

    Article • Journal of Structural Biology, April 2004, Elsevier

    Professor Bernd G Bukau, Dr. David A Dougan

  43. Targeted Delivery of an ssrA-Tagged Substrate by the Adaptor Protein SspB to Its Cognate AAA+ Protein ClpX

    Article • Molecular Cell, August 2003, Elsevier

    Professor Bernd G Bukau, Dr. David A Dougan

  44. MecA, an adaptor protein necessary for ClpC chaperone activity

    Article • Proceedings of the National Academy of Sciences, February 2003, Proceedings of the National Academy of Sciences

    Professor Bernd G Bukau, Dr. David A Dougan

  45. A folding machine for many but a master of none

    Article • Nature Structural Biology, February 2003, Springer Science + Business Media

    Professor Bernd G Bukau, Dr. David A Dougan

  46. Structural analysis of the adaptor protein ClpS in complex with the N-terminal domain of ClpA

    Article • Nature Structural Biology, November 2002, Springer Science + Business Media

    Professor Bernd G Bukau, Dr. David A Dougan

  47. Protein folding and degradation in bacteria:¶To degrade or not to degrade? That is the question

    Article • Cellular and Molecular Life Sciences, October 2002, Springer Science + Business Media

    Professor Bernd G Bukau, Dr. David A Dougan

  48. Insertion and Assembly of Human Tom7 into the Preprotein Translocase Complex of the Outer Mitochondrial Membrane

    Article • Journal of Biological Chemistry, August 2002, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr. David A Dougan

  49. AAA+ proteins and substrate recognition, it all depends on their partner in crime

    Article • FEBS Letters, August 2002, Wiley

    Professor Bernd G Bukau, Dr. David A Dougan

  50. Crystallization and preliminary X-ray analysis of theEscherichia coliadaptor protein ClpS, free and in complex with the N-terminal domain of ClpA

    Article • Acta Crystallographica Section D Biological Crystallography, June 2002, International Union of Crystallography

    Dr. David A Dougan

  51. ClpS, a Substrate Modulator of the ClpAP Machine

    Article • Molecular Cell, March 2002, Elsevier

    Professor Bernd G Bukau, Dr. David A Dougan

  52. Article • Journal of Protein Chemistry, January 1998, Springer Science + Business Media

    Dr. David A Dougan

  53. Effects of substitutions in the binding surface of an antibody on antigen affinity

    Article • Protein Engineering Design and Selection, January 1998, Oxford University Press (OUP)

    Dr. David A Dougan

  54. Heat shock proteins of barley mitochondria and chloroplasts Identification of organellar hsp 10 and 12: putative chaperonin 10 homologues

    Article • FEBS Letters, June 1992, Wiley

    Dr. David A Dougan