All Stories

  1. Insights into the Conformations of Three Structurally Diverse Proteins: Cytochrome c , p53, and MDM2, Provided by Variable-Temperature Ion Mobility Mass Spectrometry

    Article • Analytical Chemistry, March 2015, American Chemical Society (ACS)

    Dr David Clarke

  2. Desalting large protein complexes during native electrospray mass spectrometry by addition of amino acids to the working solution

    Article • The Analyst, January 2015, Royal Society of Chemistry

    Dr David Clarke

  3. Garlic Revisited: Antimicrobial Activity of Allicin-Containing Garlic Extracts against Burkholderia cepacia Complex

    Article • PLoS ONE, December 2014, PLOS

    Dr David Clarke

  4. Dissecting the Dynamic Conformations of the Metamorphic Protein Lymphotactin

    Article • The Journal of Physical Chemistry, October 2014, American Chemical Society (ACS)

    Dr David Clarke

  5. The Chemical Basis of Serine Palmitoyltransferase Inhibition by Myriocin

    Article • Journal of the American Chemical Society, September 2013, American Chemical Society (ACS)

    Dr David Clarke

  6. Serine Palmitoyltransferase K265A from S. paucimobilis with bound PLP- Myriocin Aldimine

    Article • September 2013, Protein Data Bank, Rutgers University

    Dr David Clarke

  7. WS17.3 Allicin revisited: Antimicrobial activity against the Burkholderia cepacia complex and interaction with a peroxidase target

    Article • Journal of Cystic Fibrosis, June 2013, Elsevier

    Dr David Clarke

  8. Probing the Conformational Diversity of Cancer-Associated Mutations in p53 with Ion-Mobility Mass Spectrometry

    Article • Angewandte Chemie, March 2013, Wiley

    Dr David Clarke

  9. Probing the Conformational Diversity of Cancer-Associated Mutations in p53 with Ion-Mobility Mass Spectrometry

    Article • Angewandte Chemie International Edition, March 2013, Wiley

    Dr David Clarke

  10. Redox regulation of tumour suppressor protein p53: identification of the sites of hydrogen peroxide oxidation and glutathionylation

    Article • Chemical Science, January 2013, Royal Society of Chemistry

    Dr David Clarke

  11. Reconstitution of the pyridoxal 5′-phosphate (PLP) dependent enzyme serine palmitoyltransferase (SPT) with pyridoxal reveals a crucial role for the phosphate during catalysis

    Article • Chemical Communications, January 2013, Royal Society of Chemistry

    Dr David Clarke

  12. Cellular redox potential and the biomolecular electrochemical series: A systems hypothesis

    Article • Free Radical Biology and Medicine, July 2012, Elsevier

    Dr David Clarke

  13. 72 Antimicrobial activity of stable allicin against multiresistant cystic fibrosis pathogens

    Article • Journal of Cystic Fibrosis, June 2012, Elsevier

    Dr David Clarke

  14. l-Penicillamine is a mechanism-based inhibitor of serine palmitoyltransferase by forming a pyridoxal-5′-phosphate-thiazolidine adduct

    Article • MedChemComm, January 2012, Royal Society of Chemistry

    Dr David Clarke

  15. An affinity purification procedure to isolate oxidized p53

    Article • Analytical Biochemistry, January 2012, Elsevier

    Dr David Clarke

  16. Mapping a Noncovalent Protein–Peptide Interface by Top-Down FTICR Mass Spectrometry Using Electron Capture Dissociation

    Article • Journal of the American Society for Mass Spectrometry, May 2011, American Chemical Society (ACS)

    Dr David Clarke

  17. Identification of Two Reactive Cysteine Residues in the Tumor Suppressor Protein p53 Using Top-Down FTICR Mass Spectrometry

    Article • Journal of the American Society for Mass Spectrometry, February 2011, American Chemical Society (ACS)

    Dr David Clarke

  18. The serine palmitoyltransferase from Sphingomonas wittichii RW1: An interesting link to an unusual acyl carrier protein

    Article • Biopolymers, June 2010, Wiley

    Dr David Clarke

  19. Online Quench-Flow Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Elucidating Kinetic and Chemical Enzymatic Reaction Mechanisms

    Article • Analytical Chemistry, March 2010, American Chemical Society (ACS)

    Dr David Clarke

  20. Conformational Preferences of Linear β-Defensins Are Revealed by Ion Mobility-Mass Spectrometry

    Article • The Journal of Physical Chemistry, February 2010, American Chemical Society (ACS)

    Dr David Clarke

  21. Subdivision of the Bacterioferritin Comigratory Protein Family of Bacterial Peroxiredoxins Based on Catalytic Activity

    Article • Biochemistry, February 2010, American Chemical Society (ACS)

    Dr David Clarke

  22. Binding a heparin derived disaccharide to defensin inspired peptides: insights to antimicrobial inhibition from gas-phase measurements

    Article • Physical Chemistry Chemical Physics, January 2010, Royal Society of Chemistry

    Dr David Clarke

  23. Inhibition of the PLP-dependent enzyme serine palmitoyltransferase by cycloserine: evidence for a novel decarboxylative mechanism of inactivation

    Article • Molecular BioSystems, January 2010, Royal Society of Chemistry

    Dr David Clarke

  24. Top-down protein sequencing by CID and ECD using desorption electrospray ionisation (DESI) and high-field FTICR mass spectrometry

    Article • International Journal of Mass Spectrometry, January 2010, Elsevier

    Dr David Clarke

  25. Dissection of the DNA Mimicry of the Bacteriophage T7 Ocr Protein using Chemical Modification

    Article • Journal of Molecular Biology, August 2009, Elsevier

    Dr David Clarke

  26. Dying and Necrotic Neutrophils Are Anti-Inflammatory Secondary to the Release of  -Defensins

    Article • The Journal of Immunology, July 2009, The American Association of Immunologists

    Dr David Clarke

  27. Preparation of isotopically labelled recombinant β-defensin for NMR studies

    Article • Protein Expression and Purification, June 2009, Elsevier

    Dr David Clarke

  28. Efficient Production of Human β-Defensin 2 (HBD2) in Escherichia coli

    Article • Protein and Peptide Letters, June 2009, Bentham Science Publishers

    Dr David Clarke

  29. Interrogating the Molecular Details of the Peroxiredoxin Activity of the Escherichia coli Bacterioferritin Comigratory Protein Using High-Resolution Mass Spectrometry

    Article • Biochemistry, May 2009, American Chemical Society (ACS)

    Dr David Clarke

  30. Defensin-related peptide 1 (Defr1) is allelic to Defb8 and chemoattracts immature DC and CD4+ T cells independently of CCR6

    Article • European Journal of Immunology, April 2009, Wiley

    Dr David Clarke

  31. Structural and Functional Studies of the Biotin Protein Ligase from Aquifex aeolicus Reveal a Critical Role for a Conserved Residue in Target Specificity

    Article • Journal of Molecular Biology, March 2009, Elsevier

    Dr David Clarke

  32. Plant host and sugar alcohol induced exopolysaccharide biosynthesis in the Burkholderia cepacia complex

    Article • Microbiology, August 2008, Society for General Microbiology

    Dr David Clarke

  33. Analysis and Separation of Residues Important for the Chemoattractant and Antimicrobial Activities of  -Defensin 3

    Article • Journal of Biological Chemistry, January 2008, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr David Clarke

  34. 85* Plant host and sugar alcohol induced exopolysaccharide biosynthesis in the Burkholderia cepacia complex

    Article • Journal of Cystic Fibrosis, June 2007, Elsevier

    Dr David Clarke

  35. Covalent Dimer Species of β-Defensin Defr1 Display Potent Antimicrobial Activity against Multidrug-Resistant Bacterial Pathogens

    Article • Antimicrobial Agents and Chemotherapy, March 2007, ASM Journals

    Dr David Clarke

  36. Maturation of McjA precursor peptide into active microcin MccJ25

    Article • Organic & Biomolecular Chemistry, January 2007, Royal Society of Chemistry

    Dr David Clarke

  37. Structural and functional studies of defensin-inspired peptides

    Article • Biochemical Society Transactions, April 2006, Portland Press Ltd.

    Dr David Clarke

  38. 83 Exopolysaccharide production in the Burkholderia cepacia in response to growth on onion agar

    Article • Journal of Cystic Fibrosis, January 2006, Elsevier

    Dr David Clarke

  39. Is it biologically relevant to measure the structures of small peptides in the gas-phase?

    Article • International Journal of Mass Spectrometry, February 2005, Elsevier

    Dr David Clarke

  40. Cloning, expression, purification, crystallization and preliminary X-ray characterization of the full-length single-stranded DNA-binding protein from the hyperthermophilic bacteriumAquifex aeolicus

    Article • Acta Crystallographica Section D Biological Crystallography, October 2004, International Union of Crystallography

    Dr David Clarke

  41. Structure-Activity Relationships in Defensin Dimers: A NOVEL LINK BETWEEN  -DEFENSIN TERTIARY STRUCTURE AND ANTIMICROBIAL ACTIVITY

    Article • Journal of Biological Chemistry, August 2004, American Society for Biochemistry & Molecular Biology (ASBMB)

    Dr David Clarke

  42. Biotinylation in the hyperthermophile Aquifex aeolicus. Isolation of a cross-linked BPL:BCCP complex

    Article • European Journal of Biochemistry, March 2003, Wiley

    Dr David Clarke